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. 2016 Jan 8;9:5. doi: 10.1186/s13041-016-0186-6

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© Choi et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — PINK1 deficiency did not affect GFAP mRNA expression or the signaling pathways involved in astrocyte differentiation. a, b The levels of proteins known to be involved in astrocyte differentiation-related pathways (e.g., p-SMAD1/5/8, p-STAT3, and HES1) were measured by Western blotting (a). GAPDH was used as the loading control. The band intensities of p-SMAD1/5/8, p-STAT3, and HES1 were quantified in (b). c GFAP mRNA levels were measured in WT and PINK1-KO NSCs using Q-PCR on days 0, 1, 3, and 5 of differentiation. d, e WT and PINK1-KO NSCs were differentiated in the presence of 0.1 ng/ml CNTF for 5 days. p-STAT3 levels were compared after the indicated durations of differentiation (d), and GFAP mRNA levels were examined on day 3 of differentiation (e). Values are means ± SEM of at least four samples (b, c, e)