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. Author manuscript; available in PMC: 2016 Jan 9.
Published in final edited form as: Osteoarthritis Cartilage. 2014 Apr 3;22(6):822–830. doi: 10.1016/j.joca.2014.03.017

Fig. 4.

Fig. 4

The Cx43 hemichannels opened under the FFSS but closed after fluid flow withdrawal. Ten minutes of FFSS treatment at 16 dynes/cm2 stimulated the uptake of dye in primary rat chondrocytes (A and C) and ATDC5 cells (B and D) in contrast to the untreated control cells. This increased dye uptake was blocked by 18β-GA (A and C) or Cx43 siRNA (B and D). The dye uptake capability was diminished when cells were tested after 8 h incubation following the 10 min shear stress stimulation, indicating the closure of Cx43 hemichannels after withdrawal of the FFSS stimulation. n=3 for each experiment. Values are the mean with lower and upper limits of 95% CI. *P < 0.0001. Con, control groups; FF, FFSS group.