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Two-dimensional gel electrophoresis analysis of L. pneumophila katA mutant. Isoelectric focusing (horizontal) and SDS-polyacrylamide gel electrophoresis (vertical) were performed with sonic extracts from stationary-phase (A) and Ers-treated stationary-phase (B) cultures. The regions shown are ∼20% of each gel surrounding ORF lpg2222 (arrows) (calculated molecular weight, 41,400; calculated pI 5.35). The amount of protein loaded in panel A was greater than the amount loaded in panel B.
