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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 2;1862(2):233–239. doi: 10.1016/j.bbadis.2015.12.001

Figure 4. Knockdown of HAO1 and HYPDH using siRNA in LNPs.

Figure 4

A: Western blot of GO staining compared to GAPDH in liver tissue from Wt mice 15 days post luciferase siRNA (L1) or HAO1 siRNA (L2) administration, and from Agxt KO mice treated with luciferase siRNA (L3) or HAO1 siRNA (L4).

B: Liver GO activity in Wt and Agxt KO mice 15 days post luciferase siRNA (open bars) or HAO1 siRNA (filled bars) administration. C: Western blot of HYPDH staining compared to VDAC in liver mitochondria from Wt mice 15 days post luciferase siRNA (L1) or HYPDH siRNA (L2) administration, and from Agxt KO mice treated with luciferase siRNA (L3) or HYPDH siRNA (L4).

D: Liver HYPDH activity in Wt and Agxt KO mice 15 days post luciferase (open bars) or HYPDH siRNA (filled bars) administration. ** P<0.01, *** P<0.001, for comparisons between luciferase and HAO1 or HYPDH siRNA administration using a Student t-test. Results presented are means ± SD.