FIG. 3.

Preferential binding of selected phage clones to EN sera. Microtiter plates were first coated with pooled sera (n = 10) from EN, MF, CP, or NEN subjects. Subsequently, 10⁸ clones displaying ALT-2, TPX-2, or VAH were incubated and washed, and bound phage were eluted. The numbers of bound phages were calculated by determination of titers and plotted on the y axis. Results demonstrate that recombinant phage preferentially bound to EN sera. Data presented are representative of one of five similar experiments. *, significant (P < 0.001) compared to NEN subjects.