FIG. 5.

Western blot of total parasite extract probed with rabbit antisera made against domain constructs D I+II, D II+III or D I+III. P. falciparum 3D7 schizonts were extracted with 10% SDS, subjected to SDS-PAGE under nonreducing conditions, and transferred to a nitrocellulose membrane. The blot was treated with postimmune rabbit serum against each of the three double domains, as indicated. Prebleed serum from each rabbit was negative (data not shown).