FIG. 2.

Bovine γδ T cells produce significant amounts of IFN-γ in response to M. tuberculosis cell wall (M.tbCW) when cocultured with purified monocytes and IL-2. Monocytes and γδ T cells were isolated from the peripheral blood of four animals by using magnetic beads. Monocytes and γδ T cells from each individual animal were incubated with antigens or PBS for 48 h. The supernatants were then tested for the presence of IFN-γ. The data are presented as mean OD at 450 to 650 nm ± standard error for four animals. Significant IFN-γ production was determined by one-way ANOVA with the Tukey posttest. ***, P < 0.001.