FIG. 3.

IFN-γ production by γδ T cells in response to M. bovis cell wall (CW). Magnetic beads were used to isolate monocytes and γδ T cells from the peripheral blood of 10 animals. Monocytes and γδ T cells from each individual animal were incubated with M. bovis or M. tuberculosis cell wall or PBS for 48 h. The supernatants were tested for the presence of IFN-γ. Data are presented as mean OD at 450 to 650 nm ± standard error from two successive experiments with five animals per experiment. Significant IFN-γ production was determined by one-way ANOVA with the Tukey posttest. **, P < 0.01; ***, P < 0.001.