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Published in final edited form as: Vascul Pharmacol. 2015 Jul 26;76:42–52. doi: 10.1016/j.vph.2015.07.011

A) HCAECs were maintained in EBM-2 alone (Control) or EBM-2 containing thapsigargin (“Thaps”, 1 μM, 10 min) or tunicamycin (“Tun”, 10 μg/ml, 30 min) in the presence or absence of the selective CAMKII inhibitor KN93 (“KN93”, 10 μM), and then processed for immunodetection of phospho-CAMKII (Thr286; ~50 kDa), phospho-STAT1 (Ser727; ~91 kDa) or phospho-JNK1/2 (Thr-183/Tyr-185; ~46/54 kDa) in whole cell lysates, as indicated. Membranes were reprobed for GAPDH (~37 kDa) or total STAT1 (~91 kDa) or total JNK1/2 (~46/54 kDa), as indicated, to control for protein loading. Immunodetection of CAMKII and STAT1 was always performed on the same blot, and thus the same GAPDH blot applies as a loading control for both proteins. Blots are representative of three independent experiments. B) Bar graphs show normalized densitometry values for three independent experiments. P values for the difference respect to the control are shown on top of the bars. ns: not statistically significant.

A) HCAECs were maintained in EBM-2 alone (Control) or EBM-2 containing thapsigargin (“Thaps”, 1 μM, 10 min) or tunicamycin (“Tun”, 10 μg/ml, 30 min) in the presence or absence of the selective CAMKII inhibitor KN93 (“KN93”, 10 μM), and then processed for immunodetection of phospho-CAMKII (Thr286; ~50 kDa), phospho-STAT1 (Ser727; ~91 kDa) or phospho-JNK1/2 (Thr-183/Tyr-185; ~46/54 kDa) in whole cell lysates, as indicated. Membranes were reprobed for GAPDH (~37 kDa) or total STAT1 (~91 kDa) or total JNK1/2 (~46/54 kDa), as indicated, to control for protein loading. Immunodetection of CAMKII and STAT1 was always performed on the same blot, and thus the same GAPDH blot applies as a loading control for both proteins. Blots are representative of three independent experiments. B) Bar graphs show normalized densitometry values for three independent experiments. P values for the difference respect to the control are shown on top of the bars. ns: not statistically significant.