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Published in final edited form as: Vascul Pharmacol. 2015 Jul 26;76:42–52. doi: 10.1016/j.vph.2015.07.011

A) Fura-2 loaded HEK293 cells stably overexpressing the human TRPC3 (Vazquez et al., 2004b) were maintained in nominally Ca²⁺-free medium and, when indicated, OAG (100 μM) and Ba²⁺ (2 mM) were added to the bath to evidence OAG-induced TRPC3-mediated Ba²⁺ influx. Alternatively, cells were subjected to the same protocol but in the presence of the TRPC3 selective blocker Pyr10 (2 μM) as indicated. Traces are averages of 10–20 cells and are representative of 3 independent experiments. Values for rates of Ba²⁺ influx are provided in the text. B) Fura-2 loaded HCAECs were exposed to the SERCA inhibitor thapsigargin (1 μM) in nominally Ca²⁺-free medium to deplete intracellular Ca²⁺ stores. Once the Ca²⁺ transient was over, Ca²⁺ (2 mM) was added to the bath to evidence the store-operated Ca²⁺ influx. Alternatively, cells were subjected to the same protocol but in the presence of the TRPC3 selective blocker Pyr10 (2 μM) as indicated. Traces are averages of 18–22 cells and are representative of 3 independent experiments.

A) Fura-2 loaded HEK293 cells stably overexpressing the human TRPC3 (Vazquez et al., 2004b) were maintained in nominally Ca²⁺-free medium and, when indicated, OAG (100 μM) and Ba²⁺ (2 mM) were added to the bath to evidence OAG-induced TRPC3-mediated Ba²⁺ influx. Alternatively, cells were subjected to the same protocol but in the presence of the TRPC3 selective blocker Pyr10 (2 μM) as indicated. Traces are averages of 10–20 cells and are representative of 3 independent experiments. Values for rates of Ba²⁺ influx are provided in the text. B) Fura-2 loaded HCAECs were exposed to the SERCA inhibitor thapsigargin (1 μM) in nominally Ca²⁺-free medium to deplete intracellular Ca²⁺ stores. Once the Ca²⁺ transient was over, Ca²⁺ (2 mM) was added to the bath to evidence the store-operated Ca²⁺ influx. Alternatively, cells were subjected to the same protocol but in the presence of the TRPC3 selective blocker Pyr10 (2 μM) as indicated. Traces are averages of 18–22 cells and are representative of 3 independent experiments.