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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Vascul Pharmacol. 2015 Jul 26;76:42–52. doi: 10.1016/j.vph.2015.07.011

Figure 7.

Figure 7

HCAECs were maintained in EBM-2 alone (Control) or EBM-2 containing the ER stressors thapsigargin (“Thaps”, 1 μM) or tunicamycin (“Tun”, 10 μg/ml) for 16 hours, in the presence or absence of the selective TRPC3 blocker pyrazole-10 (Pyr10, 2 μM) as indicated; treatment with Pyr10 alone was included as a control. In A), following treatments the cells were processed for immunodetection of IRE1α (~130 kDa), BiP (~75 kDa) or CHOP (~27 kDa) in whole cell lysates. Membranes were reprobed for GAPDH (~37 kDa) to control for protein loading. Blots are representative of three independent experiments. In B) the cells were processed for in vitro TUNEL assay. Where indicated HCAECs were incubated in EBM-2 containing staurosporine (“St”, 1 μM) for 16 hours, in the presence or absence of Pyr10 (2 μM). Differences between “Thaps” and “Tun” treatments and their corresponding control (“Control”) had P<0.001. Differences between staurosporine treatments and the control had P<0.001. All values are mean ± SEM (n=3–5). “ns”: not statistically significant.