Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
HCAECs were maintained in EBM-2 alone (Control) or EBM-2 containing the ER stressors thapsigargin (“Thaps”, 1 μM) or tunicamycin (“Tun”, 10 μg/ml) for 16 hours. Following treatments the cells were processed for immunodetection of TRPC3 (~100 kDa) in whole cell lysates. Membranes were reprobed for GAPDH (~37 kDa) to control for protein loading. Blots are representative of three independent experiments.
