Fig. 3.
Cartoon model of IFNG-AS1 regulation and function (human genome is shown). TH1 differentiation signals, e.g. Stat4, T-bet, etc., cooperatively activate genomic proximal and distal enhancers selective for either IFNG or IFNGAS1. T-bet and IFNG-AS1 (blue arrow) cooperate to stimulate TH1-dependent IFNG transcription; IFNG-AS1 RNA recruits H3K4 histone methyltransferases to IFNG locus helping to maintain locus in a transcriptionally favorable state. Presence of IFNG-AS1 RNA is essential for robust expression of IFNG and resistance to Salmonella infection, in vivo, in mice.