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Construction of the lrrA gene-inactivated mutant (AK-1) of strain ATCC 35405 and the lrrA gene expression plasmid (pKMlrrA). (A) The erythromycin resistance cassette (ermF-ermAM) used to inactivate the lrrA gene was inserted at the AccI site into the LRR region. (B) The lrrA gene containing a Shine-Dalgarno sequence (indicated by the arrow) was cloned into T. denticola shuttle vector pKMR4PE to construct lrrA gene expression plasmid pKMlrrA.
