FIG. 9.

Binding of the LrrA derivatives to BspA (recombinant Bsp70). Binding activities of the various LrrA derivatives were determined by enzyme-linked immunosorbent assay. Microtiter plates coated with recombinant Bsp70 were incubated with the LrrA derivatives His-LrrA, His-LrpAΔC, His-LrpAΔN, and His-LrpAΔNC for 1 h at room temperature. Bound proteins were incubated with 1:1,000-diluted anti-His-horseradish peroxidase conjugate and developed with TMB. Binding values are indicated relative to binding of His-LrrA, which was set at 100%. Values are means and standard deviations (error bars) for three separate experiments performed in duplicate.