Table 2.
Proliferation of multiple shoots in various cultivars of indica rice cultured on MS culture medium with various concentrations of growth regulators
| Growth regulators | Concentration of growth regulators (mg L−1) | Number of days taken | Average number of shoots in selected cultivarsa | |||
|---|---|---|---|---|---|---|
| A | B | C | D | |||
| MS | – | 30 | 1 | 1 | 1 | 1 |
| MS + BAP | 5.0 | 27 | 13.56 ± 0.36 | 12.49 ± 0.42 | 114.51 ± 0.11 | 11.38 ± 0.48 |
| MS + 2,4D + KIN | 0.3 + 2.1 | 29 | 12.64 ± 0.65 | 11.58 ± 0.39 | 112.53 ± 0.30 | 10.55 ± 0.72 |
| MS + 2,4D + BAP | 0.3 + 2.2 | 27 | 10.26 ± 0.48 | 08.62 ± 0.35 | 13.43 ± 0.59 | 11.23 ± 0.63 |
| MS + NAA + KIN | 2.0 + 2.1 | 28 | 10.57 ± 0.49 | 11.93 ± 0.28 | 13.11 ± 0.39 | 10.39 ± 0.51 |
| MS + NAA + BAP | 2.0 + 2.2 | 29 | 10.84 ± 0.66 | 12.54 ± 0.49 | 12.39 ± 0.49 | 12.59 ± 0.42 |
(A) PR-115, (B) PR-116, (C) Sambha mahsuri, (D) Erramallelu
Number of tubes for each concentration was five, i.e., ten for each concentration studied
Seeds showing below eight shoots were not considered
Average of number of shoots per tube was calculated and mentioned in above table
Number of shoots: Number of shoots = (A1 + A2 + A3… + A10)b = X; Number of tubes = B; Average of shoots = X ÷ B
BAP 6-Benzyl amino purine, KIN kinetin, 2,4-D 2,4-Dichlorophenoxy acetic acid, NAA 1-Naphthaleneacetic acid
aDetermination of shoots = Number of replicates for each concentration was two
bA1–A10 is number of test tubes