Fig. 2.

Phylogenetic tree of characterised bacterial hexose and pentose transporters belonging to the major facilitator superfamily. Clostridial protein sequences CacXylT (Cac1345), Cac1339, Cac1530, Cac3422 and Cac3451 of C. acetobutylicum and CbeXylT (Cbe0109) and Cbe4545 of C. beijerinckii were obtained from the C. acetobutylicum (http://www.ncbi.nlm.nih.gov/nuccore/15893298) and C. beijerinckii (http://www.ncbi.nlm.nih.gov/nuccore/150014892?report=genbank) genome websites. Other sequences included in the analysis are as follows: BloGlcP, Bifidobacterium longum GlcP WP_011068757.1 (Parche et al. 2006); BsuAraE, Bacillus subtilis AraE WP_003243899.1 (Krispin and Allmansberger 1998); BsuGlcP, B. subtilis GlcP WP_003245772.1 (Paulsen et al. 1998); CglAraE, Corynebacterium glutamicum AraE BAH60837.1 (Sasaki et al. 2009); EcoAraE, Escherichia coli AraE WP_000256438.1 (Daruwalla et al. 1981; Hasona et al. 2004); EcoFucP, E. coli FucP WP_000528603.1 (Bradley et al. 1987); EcoGalP, E. coli GalP WP_001112301.1 (Henderson et al. 1977; Hernández-Montalvo et al. 2001); EcoXylE, E. coli XylE WP_001097274.1 (Lam et al. 1980; Sun et al. 2012); LbrXylT, Lactobacillus brevis XylT O52733.1 (Chaillou et al. 1998); MsmGlcP, Mycobacterium smegmatis GlcP WP_011729622.1 (Pimentel-Schmitt et al. 2009); PanFucP, Pantoea asanatis FucP WP_013024528.1 (Andreeva et al., 2013); PanGalP, P. asanatis GalP WP_014594508.1 (Andreeva et al. 2013); PanXylE, P. asanatis XylE WP_014593545.1 (Andreeva et al. 2013); RjoGluP, Rhodococcus jostii GluP WP_009475028.1 (Araki et al. 2011); ScoGlcP, Streptomyces coelicolor GlcP WP_003971990.1 (van Wezel et al. 2005); SynGlcP, Synechocystis sp. PCC6803 GlcP WP_010873345.1 (Zhang et al. 1989); ZmoGlf, Zymomonas mobilis Glf WP_011240287.1 (Weisser et al. 1995). Sugars which have been identified as substrates are indicated as follows (D-isomers unless otherwise indicated): ara, arabinose; fru, fructose; gal, galactose; glc, glucose; man, mannose; xyl, xylose; 2DG, 2-deoxyglucose; 6DG, 6-deoxyglucose; αMG, methyl-α-glucoside. Multiple alignment of protein sequences was performed using Clustal Omega of the European Bioinformatics Institute (http://www.ebi.ac.uk/Tools/msa/clustalo/) and phylogenetic trees were drawn using TreeView (Page 1996). Scale bar = 0.1 amino acid substitution per site