FIG. 5.

(A) Representative reverse zymography of TIMP activity in alveolar macrophages stimulated with M. haemolytica products for 4 h. M, M. haemolytica supernatant; H, HTCS; C, control conditions. Purified mouse TIMPs (T) were run on the gel as standards. (B) Densitometric analysis of reverse zymography gels from four separate experiments. Columns represent the mean density (± SEM) of TIMP-1 from alveolar macrophages stimulated with CS (black), HTCS (gray), or under control conditions (white). *, significant difference from control and heat-treated sample at the same time point (P < 0.05).