FIG. 2.
Effect of sterile Aeromonas supernatant on ISC and Rt of HT-29/B6 monolayers. (A) Strain Sb aeromonads were added to the mucosal bathing solution to yield an initial concentration of 105 CFU/ml. After a 100-min latency, induction of ISC became evident. When maximum ISC values were reached, the apical bathing solution containing the bacteria (i.e., the supernatant) was removed and cleared from the aeromonads by centrifugation and sterile filtration. The sterile supernatant was then added to the mucosal compartment of new HT-29/B6 monolayers (indicated by the arrow). (B) Immediately after addition of the supernatant, the ISC started to increase and, after a short latency period, the Rt started to decrease again.