FIG. 3.
Dose response of sterile Aeromonas supernatant on ISC and Rt of HT-29/B6 monolayers. Supernatant from overnight cultures of strain Sb was added to the mucosal compartment of monolayers mounted in Ussing chambers at dilutions of up to 1:10,000. The magnitude of the maximum ISC induced (A) and the decrease in Rt (B) were clearly dependent on the concentration of the supernatant. Rt was determined for comparison 30 min after addition of the supernatant. Values are means ± the SEM of five to six monolayers for each concentration. ✽✽✽, P < 0.001 versus controls treated with carrier only.