FIG. 4.
Effect of serosal application of Aeromonas supernatant on ISC of HT-29/B6 monolayers. Supernatant (dilution 1:10) from an overnight culture of strain Sb was added to the mucosal (dotted line) or serosal (straight line) compartment of monolayers mounted in Ussing chambers as indicated. Compared to mucosal addition, serosal addition caused a reversal of the direction of the ISC induced, resulting in a drop in the basal ISC. Values are means ± the SEM of five (mucosal addition) or six (serosal addition) monolayers.