FIG. 6.
Effect of low-chloride bathing solution on Aeromonas supernatant-induced ISC. The ISC was induced by mucosal addition of Aeromonas strain Sb supernatant as described above. When submaximum ISC values (high Cl) were reached, the chloride content of the bathing solution was reduced to approximately 16 mM by repeated solution exchange. When the ISC values had stabilized after an equilibration period of 10 min (Cl reduced), chloride was added again by repeated solution exchange, and values were recorded after another 10 min of equilibration (Cl added again). Values are means ± the SEM of six monolayers. ✽✽✽, P < 0.001 versus submaximum ISC values (high Cl) recorded immediately before chloride withdrawal.