FIG. 8.
Effect of inhibitors of Aeromonas supernatant induced ISC. H8 (50 μM) is a protein kinase A inhibitor, chelerythrine (10 μM) is a protein kinase C inhibitor, genistein (185 μM) acts as a tyrosine kinase inhibitor, and BAPTA-AM (30 μM) is a membrane-permeable Ca2+ chelator. The inhibitors were given 30 min prior to the addition of the supernatant. The maximum supernatant (1:10)-induced ISC in the presence of the respective inhibitor was compared to the maximum supernatant-induced ISC in monolayers pretreated with carrier only (set as 100%). Values are means ± the SEM of five or six monolayers for each group.