TABLE 1.

Bidirectional NaCl fluxes after addition of Aeromonas supernatant in HI-29/B6 monolayers^a

Sample	Mean value (μmol h−1 cm−2) ± SEMb								Mean Rt (Ω cm2) ± SEM
	ISC	JNams	JNasm	JNanet	JClms	JClsm	JClnet	Jres
Control	0.14 ± 0.02	1.10 ± 0.09	0.93 ± 0.11	0.18 ± 0.15†	0.71 ± 0.07	0.65 ± 0.03	0.05 ± 0.07†	0.01 ± 0.16†	520 ± 14
Supernatant	1.52 ± 0.08**	2.47 ± 0.33*	1.66 ± 0.36*	0.81 ± 0.52†	2.18 ± 0.27*	3.77 ± 0.44*	−1.58 ± 0.52*	−0.87 ± 0.74†	345 ± 12**

^aStrain Sb supernatant (1:10) was added to the mucosal compartment of monolayers mounted in Ussing chambers. Under control conditions, there were no significant net fluxes. After the addition of the supernatant, all unidirectional fluxes increased significantly due to the parallel decrease in R^t. In addition, an increase of net Cl⁻ flux (i.e., Cl⁻ secretion) could be observed, whereas there were no significant changes in net Na⁺ and residual fluxes. Numerically, there was excellent agreement between the Cl⁻ secretion and the I_sc induced by the supernatant. All data are means±the SEM (n = 6 monolayers). *, P < 0.05; **, P < 0.001 (versus controls treated with carrier only for unidirectional and versus 0 for net fluxes). †, Not significantly different from zero. J, flux value; net, total.

^bJ, ion flux.