TABLE 2.
Mannitol fluxes after addition of Aeromonas supernatant in HI-29/B6 monolayersa
| Sample | Mean value ± SEM
|
|
|---|---|---|
| Jmannitolms (nmol h−1 cm−2) | Rt (Ω cm2) | |
| Control | 51 ± 0.64 | 551 ± 23 |
| Supernatant | 235 ± 8.4* | 49 ± 1.9* |
Strain Sb supernatant (1:10) was added to the mucosal compartment of monolayers mounted in Ussing chambers. After the transepithelial resistance (Rt) had dropped to <10% of its initial value, [3H]mannitol was added to the mucosal compartment, and the transepithelial permeation of the label was quantified by beta counting. Monolayers treated with carrier only served as controls. Values are means ± the SEM (n = 6 monolayers for each group). *, P < 0.001 versus controls treated with carrier only.