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Binding efficiencies of purified Amicwa 1/2a and Amicwa 4b added exogenously to L. monocytogenes bacterial cells. Equivalent numbers of either EGD (serovar 1/2a) or CHUT 82337 (serovar 4b) cells were incubated with 0, 1, 5, or 10 μg of purified Ami CWA domains. After washing, bound protein was detected by Coomassie blue staining. (A) Binding of Amicwa 1/2a. (B) Binding of Amicwa 4b.
