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Binding of Amicwa 1/2a and Amicwa 4b to eukaryotic cells. Wells coated with various concentrations of purified Amicwa 1/2a or Amicwa 4b were incubated with Caco-2 or Hep-G2 cells for 2 h at 37°C. After washing, bound cells were quantified by a colorimetric hexosaminidase assay. Values are expressed relative to the binding value obtained with poly-l-lysine-coated wells, defined arbitrarily as 100. The values are means and standard errors for two independent experiments.
