Table 1. The Oligonucleotide Primers and the Polymerase Chain Reaction Programs Used for Amplification of Urease Gene of the Ureaplasma urealyticum and 16SrRNA gene of the Mycoplasma hominis (14,15).

Target Gene	Primer Sequence (5’ - 3’)*	PCR Product, bp	PCR Programs	PCR Volume, 50µL
Urease gene of the U. urealyticum		429	1 cycle: 95 °C at 3 minutes; 30 cycle: 95 °C at 20 seconds; 58 °C at 40 seconds; 72 °C at 30 seconds.	5 µL PCR buffer 10×;1.5 mM Mgcl2; 200 µM dNTP (Fermentas); 0.5 µM of each primers F & R; 1.25 U Taq DNA polymerase (Fermentas); 2.5 µL DNA template.
F	ACGACGT CCATAAGCAACT
R	CAATCTGCTCGTGAAGTATTAC
16SrRNA gene of the M. hominis		344	1 cycle: 95 °C at 3 minutes; 30 cycle: 95 °C at 20 seconds; 58 °C at 40 seconds; 72 °C at 30 seconds; 1 cycle: 72 °C at 8 minutes	5 µL PCR buffer 10×;1.5 mM Mgcl2; 200 µM dNTP (Fermentas); 0.5 µM of each primers F & R; 1.25 U Taq DNA polymerase (Fermentas); 2.5 µL DNA template
F	CAA TGG CTA ATG CCG GAT ACG C
R	GGT ACC GTC AGT CTG CAA T