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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Neuron. 2016 Jan 6;89(1):83–99. doi: 10.1016/j.neuron.2015.12.007

Figure 4. Pharmacological inhibitors reversibly prolong progenitor prometaphase.

Figure 4

(A–F) Hoechst-stained sections from slices treated with DMSO, STLC or nocodazole (for 3 hours) fixed at +0 hours (A–C), or +3 hour washout (D–F). At 0 hours STLC and nocodazole treated progenitors accumulate in prometaphase (brown arrows) and by 3 hours anaphase cells are evident (blue arrowheads). (G) Timeline of slice culture experiment. (H) Quantification of mitoses in different conditions. Stars indicate significant differences between DMSO and STLC (red) and DMSO and nocodazole (blue) conditions at each time point. Absence of a star indicates ns. (I,J) Average spindle orientation (I) and distribution of angles (J) in DMSO treated (grey), STLC treated (black), and nocodazole treated (blue) brain slices after 3-hour (STLC) and 1-hour washout (nocodazole). (K–N) DNA FISH analysis of STLC treated brain slice (K) with higher magnification images (L,M). (N) Graph depicting lack of significant aneuploidy in STLC treated slices. ns, not significant, *P<0.05, **, P<0.01, ***, P<0.001. Scale bars: A-F: 10µm, K: 20µm, L, M: 5µm. Error bars, S.D.