Skip to main content
. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Neuron. 2016 Jan 6;89(1):83–99. doi: 10.1016/j.neuron.2015.12.007

Figure 5. Prometaphase-delay of progenitors causes ectopic neurons, reduced progenitors, and apoptosis in embryonic brain slices.

Figure 5

(A) Timeline of the slice culture experiment. (B) Quantification of the fraction of EdU+ mitotic cells in different conditions. Stars indicate significant differences between DMSO and STLC (red) and DMSO and nocodazole (blue) conditions, at each time point. (C) Paradigm for EdU pulse-chase of E13.5 brain slices. (D,J) Sections from E13.5 brain slices 14 hours after washout, using paradigm outlined in (A,C), and stained for EdU (red), CC3 (blue) and Tuj1 (green). (E-H, K-N) High magnification images of (D, J) stained for EdU (red), CC3 (white or blue), and Tuj1 (green). (I, O) High magnification images of (H, N) depicting more EdU+Tuj1+ cells (yellow) in the STLC treated brain slices versus control. (P, Q, W, X) Percentage of EdU+ cells in DMSO (grey), nocodazole (blue) and STLC (red) treated slices that are CC3+ (P), Tuj1+ (Q), Tbr2+ (W), Pax6+ (X) after 14 hour washout. (R) Percentage of CC3+EdU+ cells in each condition that are Tuj1+ neurons (black) or TuJ1- (white). (S–V) Section from E13.5 brain slices 14 hours after washout, stained for EdU (red), Pax6 (blue) and Tbr2 (green). ns, not significant, *, P<0.05, **, P<0.01, ***, P<0.001. Scale bars, D, J: 500 µm, E–I, K–O: 20 µm, S–V: 10µm. Error bars, S.D.