Table 1. The Oligonucleotide Primers and the PCR Programs Used for Amplification of the Urease Gene of the U. urealyticum and 16SrRNA Gene of the M. hominis.

Target Gene/Primer Sequence (5' - 3')	PCR Product, bp	PCR Programs	PCR Volume (50 µL)
Urease gene of the U. urealyticum	429	1 cycle: 95°C; 3 min, 30 cycle: 95°C; 20 s, 58°C; 40 s,72°C; 30 s,1 cycle: 72°C; 8 min	5 µL PCR buffer 10X; 1.5 mM Mgcl2; 200 µM dNTP (Fermentas); 0.5 µM of each primers F and R; 1.25 U Taq DNA polymerase (Fermentas); 2.5 µL DNA template
F-ACGACGT CCATAAGCAACT
R-CAATCTGCTCGTGAAGTATTAC
16S rRNA gene of the M. hominis	344	1 cycle: 95°C; 3 min, 30 cycle: 95°C; 20 s,58°C; 40 s,72°C; 30 s,1 cycle: 72°C; 8 min	5 µL PCR buffer 10X; 1.5 mM Mgcl2; 200 µM dNTP (Fermentas); 0.5 µM of each primers F and R; 1.25 U Taq DNA polymerase (Fermentas); 2.5 µL DNA template
F- CAA TGG CTA ATG CCG GAT ACG C
R-GGT ACC GTC AGT CTG CAA T