Figure 3.

A–E: Effect of microvesicles on growth rate. Different tumor cell lines (e.g., HCT116 (A), MCF7 (B), MDA-MB-231 (C), PC9 (D), and A375 (E)) were exposed to microvesicles derived from either Akti-1/2 or DMSO treated cells of the same type for 1 hour. Experiments were done in triplicate for each cell line and treatment. Error bars show the standard error of the mean (SEM). P-values shown at the top-left corner on each panel correspond to the model comparing the two slopes of the linear models fitted for each condition (i.e., DMSO or Akti-1/2 microvesicles). Non-significant p-values in all five cell lines support the finding that there is no evidence of any significant difference in cell growth based on the differential microvesicle treatment.

F–J: Akti-1/2 microvesicles bioactivity in vitro. Barplots depict the log₂ fold change (Akti1/2 - DMSO) in the total number of cells exposed to microvesicles derived from either Akti-1/2 or DMSO treated cells of the same type for 1 hour. Cells were then placed under three different stress conditions (e.g. 1% serum, 4% oxygen, standard chemotherapy agent) for 72h (grey bar). After pre-conditioning, cells were also passaged for two weeks before placing under three different stress conditions (e.g. 1% serum, 4% oxygen, standard chemotherapy agent) for 72h (open bar). Experiments were done in triplicates for each 5 different cells line (e.g., HCT116 (F), MCF7 (G), MDA-MB-231 (H), PC9 (I), and A375 (J)), treatment, and stress condition. Error bars show the standard error of the mean (SEM). Asterisks on top of bars designate statistically significant increases (i.e., p < 0.05, one-sided t-test) in cell count after exposing them to microvesicles derived from Akti-1/2 treated cells, compared to microvesicles derived from DMSO treated cells.