Figure 4. ML264 modulates expression levels of cyclins.
DLD-1 and HCT116 cells were seeded with medium containing DMSO or 10µM ML264. Twenty-four, forty-eight and seventy two hours post-treatment cells were collected for protein and RNA analysis. (A) DLD-1 and (B) HCT116 - Representative image of Western blots. (C) DLD-1 and (D) HCT116 – RNA analysis. Fold change is calculated in comparison to vehicle (DMSO) treated cells as described in Materials and Methods section.