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NSC differentiation into motor neuron lineage (HB9 staining), at day 7 and day 14, in the presence of cadmium chloride (A), mercury chloride (B), or lead acetate (C), at 10 pM to 1 nM dosages. Metal-free cultures served as controls at each time point. * indicates p < 0.05, † indicates p < 0.01, ‡ indicates p < 0.001, and no notation for p > 0.05, compared to controls.
