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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: J Immunol. 2015 Dec 14;196(2):726–737. doi: 10.4049/jimmunol.1501266

Figure 3. Myeloid cells and T-independent antigen capture are not altered in Ly9 deficient mice.

Figure 3

(A) Absolute numbers of pDCs (B220+ CD11c+), cDCs (B220− MHC−II+ CD11c+), CD8+ cDCs (CD8+ CD11b−), CD11b+ cDCs (CD8− CD11b+) and (B) numbers of metallophilic (CD11b+ MOMA-1+) and MZ (CD11b+ SIGN-R1+) macrophages in spleens from WT and Ly9−/− mice. Data shown are mean ± SEM of eight animals. (C) TNP uptake in WT and Ly9−/− MZ 30 minutes after i.v administration of 50 μg TNP-Ficoll. 5 μm frozen spleen sections were stained with anti-TNP (red), anti-SIGN-R1 (blue) and anti-IgM (green) antibodies and analysed by confocal microscopy. Image magnification 20x. (D) Representative histograms of bound TNP in MZ or follicular B cells from WT (n=4) or Ly9−/− (n=4) mice. (E) Mean fluorescence intensity of captured TNP in MZ B cells. Each dot represents an individual mouse. Data are representative of at least two independent experiments.