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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: J Immunol. 2015 Dec 14;196(2):726–737. doi: 10.4049/jimmunol.1501266

Figure 5. Anti-Ly9 mediated depletion of marginal zone and B1 B cells is maintained long-term after a single administration.

Figure 5

Mice (n=5/group) received 250 μg of Ly9.7.144 or control antibody i.p. on day 0. 26 days later, mice were sacrificed and spleen subsets were analysed by flow cytometry. (A) Proportion and (B) absolute cell numbers of transitional T1, T2 and T3 cells in spleens from anti-Ly9 or control treated mice. (C) Percentage and (D) absolute cell numbers of MZ and FO B subsets. (E) Frequencies and (F) cell numbers of spleen B1, B1a and B1b cells. (G) Total levels of IgG (left) and IgM (right) in mice serum before being treated (day 0) or 26 days after receiving a single dose of either control mAb or Ly9.7.144. Statistical significance was determined using a two-tailed paired Student’s t test. (H) Absolute cell number of B220int CD138+ plasma cells in the spleen (left) and bone marrow (right) of control or anti-Ly9 treated animals. Data are representative of two independent experiments.