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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2015 Nov 19;15(1):106–113. doi: 10.1158/1535-7163.MCT-15-0207

Figure 3.

Figure 3

A. Intracellular spatial distribution (fused fluorescence and bright field images) and flow cytometry shifts in the absence and presence of endocytosis inhibitors: (first panel) Ab-targeted vesicles (red); (second panel) urea-targeted vesicles (purple); (third panel) fluorescently labeled antibody (green), in PSMA+ HUVEC. Cell nuclei are stained in blue. Scale bar is 40 μm.

B. Corresponding quantitative intracellular distributions of Ab-targeted vesicles (red symbols), urea-based targeted vesicles (purple symbols), and the antibody (green symbols) in above incubating conditions. n indicates total number of analyzed cells. Error bars correspond to standard deviations of the means of analyzed cells.

C. Cell uptake (fused fluorescence and bright field images) and flow cytometry shifts in the absence and presence of pre-saturation of cells with the free PSMA antibody or the free lysine glutamate urea agent at 1000× excess relative to cell receptors.