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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: J Immunol. 2015 Dec 7;196(2):543–546. doi: 10.4049/jimmunol.1502080

FIGURE 4.

FIGURE 4

IL-1R-dependent signaling is required for Th17 cell differentiation. (A–C) EAU was induced in WT and Il1r−/− mice, retinae were isolated on day 21 post-immunization and analyzed by the intracellular cytokine staining assay. Shown are representative flow cytometric plots (A) and percentages of CD4+ T cells expressing IL-17 (B) and IFN-γ (C) from individual mice. (D and E) Cervical and draining lymph node cells were re-stimulated with IRBP1–20 peptide for 3 d. Amounts of IL-17 (D), IFN-γ (E), and IL-1β (F) in the culture were determined by ELISA. Data shown are from 2 independent experiments with a total of 10 mice/group.