FIGURE 5. The partial loss-of-function mutations zfh2^MS209 and zfh2^2-M390.R mitigate hypercapnia-induced reductions in egg hatching.

(A) Flies homozygous for the partial loss-of-function mutations zfh2^2-M390.R (dark grey) or zfh2^MS209 (white), or the trans-heterozygous combination of zfh2^2-M390.R and zfh2^MS209 (light grey), are not protected from the suppressive effect of 13% CO₂ on egg laying seen in control animals (w¹¹¹⁸, black). All zfh2 genotypes are w¹¹¹⁸; zfh2. (n= 998 for w¹¹¹⁸, 243 for zfh2^2-M390.R, 440 for zfh2^{2-M390.R/MS209}, 195 for zfh2^MS209)

(B, C) Hypercapnic suppression of egg hatching is attenuated in zfh2 mutants flies. **, p<0.01 using Student's t-test compared to w¹¹¹⁸ for each condition. (For B, n= 821 for w¹¹¹⁸, 488 for zfh2^2-M390.R, 408 for zfh2^{2-M390.R/MS209}, 130 for zfh2^MS209; for C, n= 845 for w¹¹¹⁸, 682 for zfh2^2-M390.R, 350 for zfh2^{2-M390.R/MS209}, 207 for zfh2^MS209)