Single colorimetric ISH assay for VGluT2 ISH in AC centered on A1 (left column), MGB (middle column), and IC (right column) at P7, P11, P14, P21, and adult. Coronal sections. Roman numerals indicate layers (sp subplate). Scale bars 250 μm in all panels. d dorsal division of MGB, v ventral division of MGB, DC dorsal cortex, IC inferior colliculus, ICc central nucleus, LC lateral cortex, PAG periaqueductal gray