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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Brain Struct Funct. 2015 Jul 10;221(5):2619–2673. doi: 10.1007/s00429-015-1062-3

Fig. 8.

Fig. 8

Quadruple FISH assay in a coronal section of adult mouse centered on A1 (from Fig. 7). a The image montages obtained at 40× in a show combined expression for VGAT, GAPDH, VGluT1, and VGluT2 mRNA, counterstained by DAPI. Subpanels 1–8 show 100× image stacks taken at sites in different layers of A1, as indicated in the composite image (left). b–g Higher-resolution examples of transcript labeling from subpanel 4 shown separately for each gene. Scale bars a 250 μm, all other panels 20 μm