. Author manuscript; available in PMC: 2017 Jun 1.

Published in final edited form as: Brain Struct Funct. 2015 Jul 10;221(5):2619–2673. doi: 10.1007/s00429-015-1062-3

Table 3.

Analysis of variance (ANOVA) comparing differences in expression levels across age (P7 to adult) by brain region and gene for RNAseq (mean normalized read counts) and ISH (normalized grayscale intensity) assays

	VGAT	VGluT1	VGluT2
RNASeq
A1	F(3,20) = 10.437, p < 0.001	F(3,20) = 102.848, p < 0.001	F(3,20) = 23.083, p < 0.001
MGB	F(3,18) = 3.169, p = 0.050	F(3,18) = 15.062, p < 0.001	F(3,18) = 3.714, p = 0.031
ISH
A1	F(4,25) = 0.766, p = 0.557	F(4,25) = 28.169, p < 0.001	F(4,25) = 7.206, p = 0.001
IC	F(4,25) = 1.905, p = 0.141	F(4,25) = n/a	F(4,25) = 14.960, p < 0.001
MGd	F(4,25) = n/a	F(4,25) = 10.343, p < 0.001	F(4,25) = 5.211, p = 0.003
MGv	F(4,25) = n/a	F(4,25) = 16.043, p < 0.001	F(4,25) = 7.054, p = 0.001

F(x,y) = z, where x is degrees of freedom for between-groups comparison, y for within groups, and z is the F statistic. n/a denotes that no measurements were made due to absence of reactivity