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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Insect Biochem Mol Biol. 2015 Nov 23;68:52–63. doi: 10.1016/j.ibmb.2015.11.001

Figure 4. MALDI-TOF MS based activity assays of recombinant fucosyltransferases.

Figure 4

After 3 days of expression, P. pastoris supernatants were tested in MALDI-TOF MS based assays for fucosyltransferase activities. (A–G) Incubation of selected dabsylated glycopeptides (dabsyl-GENR; Gly-Glu-Asn-Arg) with the recombinant enzyme preparations was performed overnight in absence (A, D, F) or presence (B, C, E, G) of GDP-Fuc as donor substrate. In all cases, the recombinant fucosyltransferases transferred fucose (indicated by the Δm/z = 146; one in the case of dabysl-GENR carrying GnGn, and up to two in the case of dabsyl-GENR carrying either GalGal or βGNβGN). Asterisks indicate ions resulting from partial laser-induced breakdown of dabsyl moiety of the substrates and products (peaks of 132 mass units smaller than the parent glycopeptides).