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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Insect Biochem Mol Biol. 2015 Nov 23;68:52–63. doi: 10.1016/j.ibmb.2015.11.001

Figure 6. Enzymatic properties of recombinant mosquito FucTs.

Figure 6

In MALDI-TOF MS-based assays, activity of recombinant, purified fucosyltransferases was measured at various temperatures (A), pH values (B) and in presence of various ions (C). Reactions (A–C) were performed with selected dabsylated glycopeptides as acceptor substrates (dabsyl-GnGn for core fucosyltransferases; dabsyl-βGNβGN for Lewis-type fucosyltransferase) and GDP-Fuc as enzyme donor. Relative activity [%] was normalised based on the condition giving the highest rate of fucose transfer and all values represent averages +/− standard error.