Table I.
Substrate specificity of recombinant FucTs
| Substrate | AgFucTA | AgFucT6 | AgFucTC |
|---|---|---|---|
| GnGn | 96% | 100% | ND |
| GnGnF3 | - | ND | ND |
| GnGnF6 | 94% | - | ND |
| GalGal | ND | 1% | 59% (1F) |
| βGNβGN | 1% | 1% | 70% (2F) |
| βGNFβGNF | ND | ND | - |
Dabsylated glycopeptides were used for fucosyltransferase assays. GnGnF3, GnGnF6 and βGNFβGNF were obtained from prior incubation with purified AgFucTA, AgFucT6 and AgFucTC respectively. Relative conversion to product in % was determined by calculating peak areas obtained from MALDI-TOF MS spectra. In case of AgFucTC, either transfer of one or two fucoses residues was detected (indicated by 1F or 2F respectively). ND, not detectable; -, not performed.