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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Insect Biochem Mol Biol. 2015 Nov 23;68:52–63. doi: 10.1016/j.ibmb.2015.11.001

Table II.

Chemical and enzymatic characterisation of recombinant FucT products

dabsylated-product (recombinant enzyme; product; m/z) Hydrofluoric acid (HF) α1,6-fucosidase (bovine kidney)
AgFucTA/GnGnF3/2206 + −
AgFucT6/GnGnF6/2206 − +
AgFucTC/GalGalF/2531 + −
AgFucTC/βGNβGNF/2613 + −

Dabsylated products (GnGnF3, GnGnF6, GalGalF, βGNβGNF) obtained from incubation with recombinant fucosyltransferases (AgFucTA, AgFucT6, AgFucTC) were split and incubated with hydrofluoric acid and α1,6-fucosidase from bovine kidney respectively. Core α1,3- and Lewis-type fucose residues demonstrated sensitivity towards hydrofluoric acid treatment, whereas core α1,6-fucose was only susceptible to bovine α1,6-fucosidase. Sensitivity towards a treatment is indicated by (+).