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Promotion of strand exchange by HsRad52 with DNA containing 50% G·C base pairs. The reaction was carried out as described in Materials and Methods. Lane 1, no protein; lanes 2–5, 1.5 μM HsRad52 protein was incubated with the indicated concentrations of ssDNA, G50(-), followed by the addition of homologous duplex DNA (at 2.5 μM) and incubation for 40 min at 37°C.
