Fig. 7.

Rad52-promoted strand exchange is not mediated by a DNA-melting activity. The reaction was carried out as described in Materials and Methods. The concentration of double-stranded DNA was 2.5 μM. (A, lanes 1–4) Standard conditions, incubation at 37°C for 40 min. Contents of each reaction as indicated. Lane 2, (Het) nine transversion mismatches in G16(-) oligonucleotide as a heterologous control. (B, lanes 5–8) Contents of each reaction were the same as the corresponding lanes in A, except that a 480-fold excess of G16(-) strands, 600 μM, was added with stop solution after incubation of reaction mixtures at 37°C for 40 min.