Table 1. Effect of ScRad52 mutants on strand exchange in vitro.

	Strand exchange, %
NaCl	WT	N91A	F173A
10 mM	23	26	3
50 mM	29	0	3

Wild-type and mutant proteins (1.5 μM) were preincubated with 20 μM 83-mer (G16-) for 5-10 min at 37°C in the presence of different concentrations of NaCl. Labeled homologous duplex DNA was added at 2.5 μM, the reaction was continued for 40 min at 37°C, and strand exchange was measured as described in Materials and Methods.