Fig. 1. S-allylcysteine (SAC) inhibited dose-dependently the wound healing and invasion induced by hepatocyte growth factor (HGF) in HNE1 and HONE1 cell lines. (A) Wounds of confluent cells seeded in 24-well plates were generated using a sterile 1,000-µL pipette tip in HNE1 and HONE1 cell lines. The cells were then washed with phosphate buffered saline and treated with HGF (10 ng/mL) alone, SAC (10, 20, or 40 mM) and HGF, or SAC alone (10, 20, or 40 mM) for 24 hours. (B) Cells were seeded in a collagen-coated transwell plate and incubated with 1% fetal bovine serum medium with HGF (10 ng/mL) alone, SAC (10, 20, or 40 mM) and HGF, or SAC alone (10, 20, 40 mM) for 24 hours. After 24 hours the invading cells were stained and counted. Data shown represent the mean ± standard deviation. NS, not significant. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.